Produced in BALB/c mice using highly pure recombinant Human IP-10 as the immunizing antigen. This antibody was purified from cell culture by Protein A affinity chromatography.
Form:
A sterile filtered antibody solution was lyophilized from PBS.
Antibody Type:
Monoclonal Antibody
Application Notes:
WesternBlot: To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml (non-reducing) or at a concentration of 1.0-2.0 µg/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.. Sandwich: In a sandwich ELISA (assuming 100 µl/well), a concentration of 1.0-2.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IP-10 when used with BioGems Biotinylated Antigen Affinity Purified anti-Human IP-10 (60-196BT) as the detection antibody at a concentration of approximately 0.25 µg/ml.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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