Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hI-TAC. Anti-Human I-TAC specific antibody was purified by affinity chromatography employing immobilized hI-TAC matrix.
Form:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Application Notes:
WesternBlot: To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect hI-TAC by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human I-TAC (60-197BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hI-TAC.. Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.125 µg/mL- 0.25 µg/mL with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.. Neutralization: To yield one-half maximal inhibition [ND<span style=font-size: 16px,>50] of the biological activity of hI-TAC (100 ng/ml), a concentration of 7.0-8.0 µg/ml of this antibody is required.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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