Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mM-CSF. Anti-Murine M-CSF specific antibody was purified by affinity chromatography employing immobilized mM-CSF matrix.
Form:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Application Notes:
WesternBlot: To detect mM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect mM-CSF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Murine M-CSF (61-091BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mM-CSF.. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mM-CSF (1.5 ng/ml), a concentration of 0.05 - 0.08 µg/ml of this antibody is required.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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