Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIA-2 (human MIA-2). Anti-Human MIA-2 specific antibody was purified by affinity chromatography and then biotinylated.
Form:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Application Notes:
WesternBlot: To detect Human MIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human MIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. . Sandwich: To detect Human MIA-2 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems Polyclonal Anti- Human MIA-2 (60-220P) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIA-2.. Direct: To detect Human MIA-2 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIA-2.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
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