ACTN4 Antibody - N-terminal region
Catalog Number:
ASB-ARP42202_T100
- Images (4)
| Article Name: | ACTN4 Antibody - N-terminal region |
| Biozol Catalog Number: | ASB-ARP42202_T100 |
| Supplier Catalog Number: | ARP42202_T100 |
| Alternative Catalog Number: | ASB-ARP42202_T100-25UL,ASB-ARP42202_T100-100UL |
| Manufacturer: | Aviva |
| Host: | Rabbit |
| Category: | Proteine/Peptide |
| Application: | IHC, WB |
| Species Reactivity: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN4 |
| Alternative Names: | FSGS, FSGS1, ACTININ-4 |
| Alpha actinins belong to the spectrin superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN4 is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in its gene have been associated with focal and segmental glomerulosclerosis.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. |
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Sample Type: ACTNX-GFP transfected COS-7 cellsPrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-Alexa-Fluor 568Secondary Antibody Dilution: 1:100Color/Signal Descriptions: Green: GFP Red: ACTN4Gene Name: ACTN4Submitted by: Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710 |
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WB Suggested Anti-ACTN4 Antibody Titration: 0.625ug/ml Positive Control: HCT116 cell lysateACTN4 is supported by BioGPS gene expression data to be expressed in HCT116 |
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Lanes: Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate Primary Antibody Dilution: 1: 1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: ACTN4 Submitted by: Johannes W. Hell, University of California |
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ACTN4 antibody - N-terminal region (ARP42202_T100) in Human HCT116 using Western Blot |
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