UBE2D1 Antibody - middle region

Catalog Number: ASB-ARP43006_T100
Article Name: UBE2D1 Antibody - middle region
Biozol Catalog Number: ASB-ARP43006_T100
Supplier Catalog Number: ARP43006_T100
Alternative Catalog Number: ASB-ARP43006_T100-25UL,ASB-ARP43006_T100-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: WB
Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2D1
Alternative Names: SFT, UBCH5, UBC4/5, UBCH5A, E2(17)KB1
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Clonality: Polyclonal
Concentration: 1.0 mg/ml
Molecular Weight: 16kDa
NCBI: 7321
UniProt: P51668
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
WB Suggested Anti-UBE2D1 Antibody Titration: 1.25ug/ml
Positive Control: Jurkat cell lysate
Lanes:
1: 40ng HIS-UBE2D1 protein
2: 40ng HIS-UBE2D2 protein
3: 40ng HIS-UBE2D3 protein
4: 40ng HIS-UBE2D4 protein
5: 40ng HIS-UBE2E1 protein
6: 40ng HIS-UBE2E2 protein
7: 40ng HIS-UBE2E3 protein
8: 40ng HIS-UBE2K protein
9: 40ng HIS-UBE2L3 protein
10: 40ng HIS-UBE2N protein
11: 40ng HIS-UBE2V1 protein
12: 40ng HIS-UBE2V2 protein.
Primary Antibody Dilution:
1:500
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:50,000
Gene Name:
UBE2D1
Submitted by:
Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.
Host: Rat
Target Name: UBE2D1
Sample Tissue: Rat Skeletal Muscle
Antibody Dilution: 1ug/ml
UBE2D1 antibody - middle region (ARP43006_T100) in Human Jurkat using Western Blot