RNF128 Antibody - C-terminal region
Catalog Number:
ASB-ARP43311_T100
- Images (4)
| Article Name: | RNF128 Antibody - C-terminal region |
| Biozol Catalog Number: | ASB-ARP43311_T100 |
| Supplier Catalog Number: | ARP43311_T100 |
| Alternative Catalog Number: | ASB-ARP43311_T100-25UL,ASB-ARP43311_T100-100UL |
| Manufacturer: | Aviva |
| Host: | Rabbit |
| Category: | Proteine/Peptide |
| Application: | WB |
| Species Reactivity: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat |
| Immunogen: | The immunogen is a synthetic peptide directed towards the C terminal region of human RNF128 |
| Alternative Names: | GRAIL |
| RNF128 is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of RNF128 in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of RNF128 was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype.The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. |
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WB Suggested Anti-RNF128 Antibody Titration: 1.25ug/ml Positive Control: HepG2 cell lysateRNF128 is supported by BioGPS gene expression data to be expressed in HepG2 |
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Lanes: 1: CD4 T cell lysate from WT mice, 2: CD4 T cell lysate from WT mice treated with anti-CD3/CD28, 3: CD4 T cell lysate from WT mice treated with FK506, 4: CD4 T cell lysate from WT mice treated with anti-CD3/CD28 and FK506, 5: CD4 T cell lysate from TRPV1 KO mice, 6: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28, 7: CD4 T cell lysate from TRPV1 KO mice treated with FK506, 8: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28 and FK506. Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:10,000 Gene Name: RNF128 Submitted by: Christine Quinley, UCSD |
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25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. |
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RNF128 antibody - C-terminal region (ARP43311_T100) in Human HepG2 using Western Blot |
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