CAD Antibody - N-terminal region

Catalog Number: ASB-ARP46105_P050
Article Name: CAD Antibody - N-terminal region
Biozol Catalog Number: ASB-ARP46105_P050
Supplier Catalog Number: ARP46105_P050
Alternative Catalog Number: ASB-ARP46105_P050-25UL,ASB-ARP46105_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CAD
Alternative Names: CDG1Z, DEE50, GATD4, EIEE50
CAD is a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 243kDa
NCBI: 790
UniProt: P27708
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
WB Suggested Anti-CAD Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 293T cell lysate
Lanes:
1. 45ug capan1 cell lysate
2. 45 ug HPAF cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
CAD
Submitted by:
Dr. Pankaj Singh, UNMC, Omaha, NE
CAD antibody - N-terminal region (ARP46105_P050) in Human 293T using Western Blot