ETF1 Antibody - N-terminal region

Catalog Number: ASB-ARP54738_P050
Article Name: ETF1 Antibody - N-terminal region
Biozol Catalog Number: ASB-ARP54738_P050
Supplier Catalog Number: ARP54738_P050
Alternative Catalog Number: ASB-ARP54738_P050-25UL,ASB-ARP54738_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: IHC, WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ETF1
Alternative Names: ERF, RF1, ERF1, TB3-1, D5S1995, SUP45L1
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 49kDa
NCBI: 2107
UniProt: P62495
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Rabbit Anti-ETF1 Antibody
Catalog Number: ARP54738_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in bile ductule
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
WB Suggested Anti-ETF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: THP-1 cell lysate
ETF1 antibody - N-terminal region (ARP54738_P050) in Human THP-1 using Western Blot