SPRR1B Antibody - middle region
Catalog Number:
ASB-ARP63557_P050
- Images (4)
| Article Name: | SPRR1B Antibody - middle region |
| Biozol Catalog Number: | ASB-ARP63557_P050 |
| Supplier Catalog Number: | ARP63557_P050 |
| Alternative Catalog Number: | ASB-ARP63557_P050-25UL,ASB-ARP63557_P050-100UL |
| Manufacturer: | Aviva |
| Host: | Rabbit |
| Category: | Proteine/Peptide |
| Application: | IHC, WB |
| Species Reactivity: | Bovine, Equine, Human, Mouse, Porcine, Rabbit, Rat |
| Alternative Names: | SPRR1, GADD33, SPR-IB, CORNIFIN |
| SPRR1B is a cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. SPRR1B can function as both amine donor and acceptor in transglutaminase-mediated cross-linkage. |
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Researcher: Dr. Cintia S. De Paiva, Baylor College of Medicine Application: IHC Species+tissue/cell type:Mouse eyeball section Primary antibody dilution: 1:50 Secondary antibody: Goat anti-rabbit-AlexaFluor 488 Secondary antibody dilution:1:50 |
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WB Suggested Anti-SPRR1B Antibody Titration: 1.0 ug/ml Positive Control: Jurkat Whole Cell |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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SPRR1B antibody - middle region (ARP63557_P050) in Human Jurkat using Western Blot |
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