SFTPA2 Antibody - N-terminal region

Article Name:	SFTPA2 Antibody - N-terminal region
Biozol Catalog Number:	ASB-ARP64034_P050
Supplier Catalog Number:	ARP64034_P050
Alternative Catalog Number:	ASB-ARP64034_P050-25UL,ASB-ARP64034_P050-100UL
Manufacturer:	Aviva
Host:	Rabbit
Category:	Proteine/Peptide
Application:	WB
Species Reactivity:	Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish
Alternative Names:	PSAP, PSPA, SP-A, SPA2, PSP-A, SFTP1, SP-2A, SPAII, COLEC5, SFTPA2B

This gene is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10. Mutations in this gene and a highly similar gene located nearby, which affect the highly conserved carbohydrate recognition domain, are associated with idiopathic pulmonary fibrosis. The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication.

Clonality:	Polyclonal
Concentration:	0.5 mg/ml
Molecular Weight:	26kDa
NCBI:	729238
UniProt:	Q8IWL1
Form:	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

	WB Suggested Anti-SFTPA2 Antibody Titration: 1.0 ug/ml Positive Control: Fetal Heart
	Lanes: 1: 20ng human SP-A2 protein, 2: 20ug rat wt BAL cell lysate, 3: 25ng hSP-A2 (1A0) protein purified from transfected CHO lysate, 4: 25ng hSP-A2 (1A1) protein purified from transfected CHO lysate, 5: 25ng hSP-A1 (6A2) protein purified from transfected CHO lysate, 6: 25ng hSP-A1 (6A4) protein purified from transfected CHO lysate, 7: 25ng hSP-A2/1 (1A0/6A4) protein purified from transfected CHO lysate, 8: 25ng hSP-A2/1 (1A1/6A2) protein purified from transfected CHO lysate, 9: 20ug SP-A KO mouse BAL lysate, 10: 25ng hSP-A2 (1A0) protein purified from transfected mouse BAL lysate, 11: 25ng hSP-A2 (1A1) protein purified from transfected mouse BAL lysate, 12: 25ng hSP-A1 (6A2) protein purified from transfected mouse BAL lysate, 13: 25ng hSP-A1 (6A4) protein purified from transfected mouse BAL lysate, 14: 25ng hSP-A2/1 (1A0/6A2) protein purified from transfected mouse BAL lysate, 15: 25ng mouse SP-A2 protein purified from mouse BAL lysate,16: 20ug mouse wt BAL lysate, 17: 15ug human wt T2 lysate, 18: 25ng human SP-A2 protein.
	Lanes: Lane 1: 25ng purified Human Alveolar sample (w/SP-A1+SP-A2) Lane 1: 25ng purified Human Alveolar sample (w/SP-A1+SP-A2) Lane 2: 20ug Rat bronchoalveolar lavage Lane 3: 25ng hSP-A2 variant expressed in CHO cells Lane 4: 25ng hSP-A2 variant expressed in CHO cells Lane 5: 25ng hSP-A1/A2 variants expressed in CHO cells Lane 6: 25ng hSP-A1/A2 variants expressed in CHO cells Lane 7: 20ug SP-A1/2 KO mouse bronchoalveolar lavage Lane 8: 20ug hSP-A2 variant transgenic mouse bronchoalveolar lavage Lane 9: 20ug hSP-A2 variant transgenic mouse bronchoalveolar lavage Lane 10: 20ug hSP-A1 variant transgenic mouse bronchoalveolar lavage Lane 11: 20ug hSP-A1 variant transgenic mouse bronchoalveolar lavage Lane 12: 20ug hSP-A1/A2 variants transgenic mouse bronchoalveolar lavage Lane 13: 20ug mSP-A1/A2 bronchoalveolar lavage, +/- mouse Lane 14: 20ug mSP-A1/A2 bronchoalveolar lavage, WT mouse Lane 15: 10ug human alveolar cell lysate Lane 16: 25ng purified hSP-A1/A2 Primary Antibody Dilution: 1:2500 Secondary Antibody: IgG HRP Conj Secondary Antibody Dilution: 1:10000 Gene Name: SFTPA2 Submitted by: Todd M. Umstead, Pennsylvania State University College of Medicine
	SFTPA2 antibody - N-terminal region (ARP64034_P050) in Human Fetal heart using Western Blot