Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
Immunogen:
The immunogen is a synthetic peptide directed towards the C-terminal region of Human COL6A5
Alternative Names:
VWA4, COL29A1
The protein encoded by this gene is a collagen that contains N- and C-terminal von Willebrand factor A-like domains. The encoded protein may interact with the alpha 1 and alpha 2 chains of collagen VI to form the complete collagen VI trimer. Sequence polymorphisms in this gene have been linked to atopic dermatitis (eczema). Two transcript variants, one protein-coding and the other probably not protein-coding, have been found for this gene.
Host: Rabbit Target: COL6A5 Positive control (+): Human Lung (LU) Negative control (-): Human Placenta (PL) Antibody concentration: 1ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
COL6A5 Antibody - C-terminal region (ARP67053_P050) in Human Fetal heart using Western Blot
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