MYLK3 Antibody - C-terminal region

Catalog Number: ASB-ARP69121_P050
Article Name: MYLK3 Antibody - C-terminal region
Biozol Catalog Number: ASB-ARP69121_P050
Supplier Catalog Number: ARP69121_P050
Alternative Catalog Number: ASB-ARP69121_P050-25UL,ASB-ARP69121_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human
Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human MYLK3
Alternative Names: MLCK, MLCK2, caMLCK
Phosphorylation of cardiac myosin heavy chains and light chains by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 88kDa
NCBI: 91807
UniProt: Q32MK0
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Host: Rabbit
Target Name: MYLK3
Sample Type: HepG2 Whole Cell lysates
Antibody Dilution: 1.0ug/ml
Host: Rabbit
Target: MYLK3
Positive control (+): HeLa Cell Lysate (HL)
Negative control (-): Human Placenta (PL)
Antibody concentration: 1ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
MYLK3 Antibody - C-terminal region (ARP69121_P050) in Human HepG2 using Western Blot