The immunogen is a synthetic peptide directed towards the C-terminal region of Human MYLK3
Alternative Names:
MLCK, MLCK2, caMLCK
Phosphorylation of cardiac myosin heavy chains and light chains by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes.
Host: Rabbit Target: MYLK3 Positive control (+): HeLa Cell Lysate (HL) Negative control (-): Human Placenta (PL) Antibody concentration: 1ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
MYLK3 Antibody - C-terminal region (ARP69121_P050) in Human HepG2 using Western Blot
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