GRIA2 Antibody - N-terminal region

Catalog Number: ASB-AVARP13038_T100
Article Name: GRIA2 Antibody - N-terminal region
Biozol Catalog Number: ASB-AVARP13038_T100
Supplier Catalog Number: AVARP13038_T100
Alternative Catalog Number: ASB-AVARP13038_T100-25UL,ASB-AVARP13038_T100-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: IHC, WB
Species Reactivity: Bovine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GRIA2
Alternative Names: GLUR2, GLURB, GluA2, HBGR2, NEDLIB, gluR-2, gluR-B, GluR-K2
GRIA2 is one of the glutamate receptors, which are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with mul
Clonality: Polyclonal
Concentration: 1.0 mg/ml
Molecular Weight: 99kDa
NCBI: 2891
UniProt: P42262
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Primary AntibodiesBrain slices were incubated overnight in a solution of PBST (0.2%) + 10% Normal GOAT serum + a monoclonal mouse anti-Parvalbumin antibody (1:2000)+ rabbit anti-GRIA2 (Aviva systems Biology, 1ug/mL)and gently shaken overnight at 4 C. Secondary AntibodiesSamples were washed 6x in PBS (10 min each at RT) and exposed to the following secondary antibodies o/n at 4 C: PBST (0.3%) + 10% normal goat serum + anti-mouse Alexa 488 (1:1000) + Anti-rabbit Alexa 555 (1:1000) for two hours. Samples were washed 6x in PBST mounted. Images acquired with a confocal microscope at 63X.
Application: Immunohistochemistry
Species+tissue/cell type: Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop
How many ugsof tissue/cell lysate run on the gel: 11 m Mouse Gut Tissue
Primary antibody dilution: 1:50
Secondary antibody: Goat anti-rabbit-cy3
Secondary antibody dilution: 1:1500
WB Suggested Anti-GRIA2 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysate
GRIA2 antibody - N-terminal region (AVARP13038_T100) in Human HepG2 using Western Blot