DDX11 Antibody

Article Name:	DDX11 Antibody
Biozol Catalog Number:	ASB-OACA08684
Supplier Catalog Number:	OACA08684
Alternative Catalog Number:	ASB-OACA08684-100UL,ASB-OACA08684-50UL
Manufacturer:	Aviva
Host:	Rabbit
Category:	Proteine/Peptide
Application:	ELISA, IHC, WB
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant Human ATP-dependent DNA helicase DDX11 protein (1-220AA)
Conjugation:	Unconjugated
Alternative Names:	ATP-dependent DNA helicase DDX11,CHL1,CHL1-like helicase homolog,CHL1-related helicase gene-1,CHL1-related protein 1,CHLR1,DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11,DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae),DEAD/H box protein 11,DEAD/H-box protein 11,hCHLR1,keratinocyte growth factor-regulated gene 2 protein,KRG2,KRG-2,probable ATP-dependent DNA helicase DDX11,probable ATP-dependent RNA helicase DDX11,WABS.

DNA-dependent ATPase and ATP-dependent DNA helicase that participates in various functions in genomic stability, including DNA replication, DNA repair and heterochromatin organization as well as in ribosomal RNA synthesis (PubMed:10648783, PubMed:21854770, PubMed:23797032, PubMed:26089203, PubMed:26503245). Its double-stranded DNA helicase activity requires either a minimal 5-single-stranded tail length of approximately 15 nt (flap substrates) or 10 nt length single-stranded gapped DNA substrates of a partial duplex DNA structure for helicase loading and translocation along DNA in a 5 to 3 direction (PubMed:18499658, PubMed:22102414). The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended up to 500 bp by the replication protein A (RPA) or the cohesion CTF18-replication factor C (Ctf18-RFC) complex activities (PubMed:18499658). Shows also ATPase- and helicase activities on substrates that mimic key DNA intermediates of replication, repair and homologous recombination reactions, including forked duplex, anti-parallel G-quadruplex and three-stranded D-loop DNA molecules (PubMed:22102414, PubMed:26503245). Plays a role in DNA double-strand break (DSB) repair at the DNA replication fork during DNA replication recovery from DNA damage (PubMed:23797032). Recruited with TIMELESS factor upon DNA-replication stress response at DNA replication fork to preserve replication fork progression, and hence ensure DNA replication fidelity (PubMed:26503245). Cooperates also with TIMELESS factor during DNA replication to regulate proper sister chromatid cohesion and mitotic chromosome segregation (PubMed:17105772, PubMed:18499658, PubMed:20124417, PubMed:23116066, PubMed:23797032). Stimulates 5-single-stranded DNA flap endonuclease activity of FEN1 in an ATP- and helicase-independent manner, and hence it may contribute in Okazaki fragment processing at DNA replication fork during lagging strand DNA synthesis (PubMed:18499658). Its ability to function at DNA replication fork is modulated by its binding to long non-coding RNA (lncRNA) cohesion regulator non-coding RNA DDX11-AS1/CONCR, which is able to increase both DDX11 ATPase activity and binding to DNA replicating regions (PubMed:27477908). Plays also a role in heterochromatin organization (PubMed:21854770). Involved in rRNA transcription activation through binding to active hypomethylated rDNA gene loci by recruiting UBTF and the RNA polymerase Pol I transcriptional machinery (PubMed:26089203). Plays a role in embryonic development and prevention of aneuploidy (By similarity). Involved in melanoma cell proliferation and survival (PubMed:23116066). Associates with chromatin at DNA replication fork regions (PubMed:27477908). Binds to single- and double-stranded DNAs (PubMed:9013641, PubMed:18499658, PubMed:22102414).

Clonality:	Polyclonal
NCBI:	1663
Form:	Liquid

	Immunohistochemistry of paraffin-embedded human gastric cancer at dilution 1:100
	Immunohistochemistry of paraffin-embedded human tonsil tissue at dilution 1:100
	Western blot All lanes: DDX11 antibody at 8.47 ug/ml Lane 1: Mouse gonadal tissue