E.coli-derived mouse Bsg recombinant protein (Position: S39-R325).
Alternative Names:
5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF
Boster Bio Anti-CD147/Bsg Antibody Picoband catalog A00248-3. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Purity:
Immunogen affinity purified.
Form:
Lyophilized
Target:
Basigin
Application Dilute:
Western blot, 0.1-0.25µg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Mouse, Rat Immunofluorescence, 5µg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3µg/1x106 cells, Mouse ELISA, 0.1-0.5µg/ml, -
IHC analysis of CD147/Bsg using anti-CD147/Bsg antibody (A00248-3). CD147/Bsg was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CD147/Bsg Antibody (A00248-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of CD147/Bsg using anti-CD147/Bsg antibody (A00248-3). CD147/Bsg was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CD147/Bsg Antibody (A00248-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of CD147/Bsg using anti-CD147/Bsg antibody (A00248-3). CD147/Bsg was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CD147/Bsg Antibody (A00248-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of CD147/Bsg using anti-CD147/Bsg antibody (A00248-3). CD147/Bsg was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CD147/Bsg Antibody (A00248-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
Western blot analysis of CD147/Bsg using anti-CD147/Bsg antibody (A00248-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147/Bsg antigen affinity purified polyclonal antibody (Catalog A00248-3) at 0.25 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for CD147/Bsg at approximately 45 kDa. The expected band size for CD147/Bsg is at 45 kDa.
IF analysis of CD147/Bsg using anti-CD147/Bsg antibody (A00248-3). CD147/Bsg was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed
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