E.coli-derived human PREP recombinant protein (Position: M1-E227).
Alternative Names:
PE, PEP, Post proline cleaving enzyme, PREP, prolyl endopeptidase
Boster Bio Anti-Prolyl Endopeptidase/PREP Antibody Picoband catalog A00298-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Purity:
Immunogen affinity purified.
Form:
Lyophilized
Target:
Prolyl endopeptidase
Application Dilute:
Western blot, 0.25-0.5µg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human, Rat Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human ELISA, 0.1-0.5µg/ml, -
IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2). Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2). Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2). Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2). Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with
Western blot analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prolyl Endopeptidase/PREP antigen affinity purified polyclonal antibody (Catalog A00298-2) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for Prolyl Endopeptidase/PREP at approximately 81 kDa. The expected band size for Prolyl Endopeptidase/PREP is at 81 kDa.
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