Boster Bio Anti-Lactoferrin/LTF Antibody Picoband catalog A00633-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Purity:
Immunogen affinity purified.
Form:
Lyophilized
Target:
Lactotransferrin
Application Dilute:
Western blot, 0.1-0.5µg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human Immunofluorescence, 5µg/ml, Human ELISA, 0.1-0.5µg/ml, -
LTF, a ferroptosis-related gene, is identified in aging cochleae. (A) The volcano diagram of shows 43385 DEGs between the old group ( ~864316) and young group ( ~864308). (B) The heatmap shows 28 statistically significant DEGs. (C) Functional analysis of the statistically significant DEGs. (D) LTF, the hub gene, is obtained from the intersection of Up-Gens, Down-Gens (based on the statistically significant DEGs), and FRGs. (E) The PPI network shows that 12 proteins are interacting with LTF.Index in PubMed under a CC BY license. PMID:
Western blot analysis of Lactoferrin/LTF using anti-Lactoferrin/LTF Antibody (A00633-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Total LTF from mouse neutrophil cell lysates. Lane 2: degranulated LTF. Ratio 1:20.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% BSA for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lactoferrin/LTF antigen affinity purified polyclonal antibody (A00633-1) at 1:2000 1h overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:3000 dilution at RT for 1 hour. The signal is developed using HRP. A specific band was detected for Lactoferrin/LTF at approximately 77 kDa. The expected band size for Lactoferrin/LTF is at 77 kDa.
IF analysis of LTF using anti-LTF antibody (A00633-1). LTF was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-LTF Antibody (A00633-1) overnight at 4C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of LTF using anti-LTF antibody (A00633-1). LTF was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-LTF Antibody (A00633-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
Western blot analysis of LTF using anti-LTF antibody (A00633-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTF antigen affinity purified polyclonal antibody (A00633-1) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog AR1196-200)with Tanon 5200 system. A specific band was detected for LTF at approximately 85 kDa. The expected band size for LTF is at 78 kDa.
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