Boster Bio Anti-VEGF Receptor 2/Kdr Antibody Picoband catalog A00901-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Flow Cytometry analysis of LLC cells using anti-VEGF Receptor 2 antibody (A00901-3).Overlay histogram showing LLC cells stained with A00901-3 (Blue line). To facilita
IF analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3). VEGF Receptor 2 was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2µg/mL rabbit anti-VEGF Receptor 2 Antibody (A00901-3) overnight at 4C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3).VEGF Receptor 2 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VEGF Receptor 2 Antibody (A00901-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3).VEGF Receptor 2 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VEGF Receptor 2 Antibody (A00901-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MarkerLane 2: mouse heart tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse kidney tissue lysates. Lane 6: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. Lane 7: mouse HEPA1-6 whole cell lysates,Lane 8: mouse NIH3T3 whole cell lysates.The membrane was incubated with rabbit anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog A00901-3) at 1.0 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system.
Flow Cytometry analysis of MFC cells using anti-VEGF Receptor 2 antibody (A00901-3).Overlay histogram showing MFC cells stained with A00901-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGF Receptor 2 Antibody (A00901-3,1µg/1x106 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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