E.coli-derived human MCK10/NEP/DDR1 recombinant protein (Position: R341-A909).
Alternative Names:
CAK, CD167, Cell adhesion kinase, DDR, DDR1, EDDR1, HGK2, Mammary carcinoma kinase 10, MCK 10, MCK10, NEP, NTRK4, Protein tyrosine kinase 3A, Protein tyrosine kinase RTK 6, PTK3, PTK3A, RTK6, TRK E, TRKE, Tyrosine kinase DDR, Tyrosine protein kinase CAK
Boster Bio Anti-MCK10/NEP/DDR1 Antibody Picoband catalog A00905. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Western blot, 0.25-0.5µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human Flow Cytometry (Fixed), 1
IHC analysis of DDR1 using anti-DDR1 antibody (A00905). DDR1 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, ep
Western blot analysis of DDR1 using anti-DDR1 antibody (A00905). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates&44, Lane 2: human A549 whole cell lysates&44, Lane 3: human K562 whole cell lysates&44, Lane 4: human HepG2 whole cell lysates&44, Lane 5: human PC-3 whole cell lysates&44, Lane 6: human THP-1 whole cell lysates. After Electrophoresis&44, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR1 antigen affinity purified polyclonal antibody (Catalog A00905) at 0.5 µg/mL overnight at 4C&44, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for DDR1 at approximately 101KD. The expected band size for DDR1 is at 101KD.
Western blot analysis of DDR1 using anti-DDR1 antibody (A00905). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates&44, Lane 2: rat lung tissue lysates&44, Lane 3: rat testicular tissue lysates&44, Lane 4: mouse brain tissue lysates&44, Lane 5: mouse lung tissue lysates&44, Lane 6: mouse testicular tissue lysates. After Electrophoresis&44, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR1 antigen affinity purified polyclonal antibody (Catalog A00905) at 0.5 µg/mL overnight at 4C&44, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for DDR1 at approximately 101KD. The expected band size for DDR1 is at 101KD.
IHC analysis of DDR1 using anti-DDR1 antibody (A00905). DDR1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDR1 Antibody (A00905) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of DDR1 using anti-DDR1 antibody (A00905). DDR1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDR1 Antibody (A00905) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
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