Anti-Integrin linked ILK Antibody Picoband, Rabbit, Polyclonal
Biozol Catalog Number:
BOB-A02932-3
Supplier Catalog Number:
A02932-3
Alternative Catalog Number:
BOB-A02932-3-100UG
Manufacturer:
Boster Bio
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, FC, ICC, IF, IHC, WB
Species Reactivity:
Human, Mouse, Rat
Immunogen:
E.coli-derived human Integrin linked ILK recombinant protein (Position: M1-K452).
Alternative Names:
ILK, ILK 1, ILK 2, ILK1, ILK2, P59, p59ILK
Boster Bio Anti-Integrin linked ILK Antibody Picoband catalog A02932-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human, Mouse, Rat ELISA, 0.1-0
IHC analysis of Integrin Linked ILK using anti-Integrin Linked ILK antibody (A02932-3). Integrin Linked ILK was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Integrin Linked ILK Antibody (A02932-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of Integrin Linked ILK using anti-Integrin Linked ILK antibody (A02932-3). Integrin Linked ILK was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Integrin Linked ILK Antibody (A02932-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of Integrin Linked ILK using anti-Integrin Linked ILK antibody (A02932-3). Integrin Linked ILK was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Integrin Linked ILK Antibody (A02932-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of Integrin Linked ILK using anti-Integrin Linked ILK antibody (A02932-3). Integrin Linked ILK was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Integrin Linked ILK Antibody (A02932-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Comple
Western blot analysis of Integrin Linked ILK using anti-Integrin Linked ILK antibody (A02932-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Linked ILK antigen affinity purified polyclonal antibody (Catalog A02932-3) at 0.25 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for Integrin Linked ILK at approximately 51 kDa. The expected band size for Integrin Linked ILK is at 51 kDa.
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