E.coli-derived human PYGM recombinant protein (Position: V390-R804). Human PYGMshares 95.9% and 94.2% amino acid (aa) sequence identity with mouse and rat PYGM, respectively.
Alternative Names:
PYGM, Myophosphorylase, EC:2.4.1.1
Boster Bio Anti-PYGM Antibody Picoband catalog A02971-1. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 µg/ml, Human Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human ELISA, 0.1-0.5 µg/ml
IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-PYGM Antibody (A
Western blot analysis of PYGM using anti-PYGM antibody (A02971-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates,Lane 2: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYGM antigen affinity purified polyclonal antibody (A02971-1) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog AR1196-200) with Tanon 5200 system. A specific band was detected for PYGM at approximately 97 kDa. The expected band size for PYGM is at 97 kDa.
IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
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