A synthetic peptide corresponding to a sequence in the middle region of human YY1, identical to the related mouse sequence.
Alternative Names:
DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor
Boster Bio Anti-YY1 Antibody Picoband catalog PB9909. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugatio
Purity:
Immunogen affinity purified.
Form:
Lyophilized
Target:
Transcriptional repressor protein YY1
Application Dilute:
Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human
YY1 + macrophages accumulate in hypoxic tumor tissues. a Schematic diagram showing the process of imaging mass cytometry (IMC) using prostate tissue. IMC of the indicated markers in prostate cancer (PCa) patient tumors ( b ) and para-cancerous tissues ( c ). Pathchs were defined as containing at least 10 cells with a maximum distance of 15µm between cells involved. Adjacent regions were merged together for downstream analysis. The expression of the marker was normalized using z -score, and a threshold of 0.5 was selected. Dot plots showing the ratio of YY1 + or YY1 - macrophages and the relative YY1 expression of macrophages in HIF-1alpha + or HIF-1alpha - patches. The representative plot and analysis are based on 46 tumor samples and 26 para-cancerous tissues. Dot plots present the mean valuesSD, and the p values are calculated using a two-tailed t -test. The p values of left, middle, and right plots of ( b ) are 0.002, 0.160, and 0.009, respectively, The p values of left, middle, and right plots of ( c ) are 0.759, 0.798, and 0.820, respectively. * P <0.05, ns P >0.05. Source data are provided as a file. M macrophage. Index in PubMed under a CC BY license. PMID: 40623999
Hypoxia e
IF analysis of YY1 using anti-YY1 antibody (PB9909). YY1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2µg/mL rabbit anti-YY1 Antibody (PB9909) overnight at 4C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Flow Cytometry analysis of THP-1 cells using anti-YY1 antibody (PB9909). Overlay histogram showing THP-1 cells stained with PB9909 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YY1 Antibody (PB9909&44,1µg/1x106 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&44, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of YY1 using anti-YY1 antibody (PB9909). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YY1 antigen affinity purified polyclonal antibody (Catalog PB9909) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002)with Tanon 5200 system. A specific band was detected for YY1 at approximately 65 kDa. The expected band size for YY1 is at 45 kDa.
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