NFQ1, a non-fluorescent quencher, is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method employing a novel, non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond. After the quenched donor emission is quantitated the acceptor is removed by reduction, enabling measurement of unquenched donor emission in the same sample. &lamba,Abs: 474 nm Orange solid soluble in DMSO at 20 mM Store desiccated at -20C and protect from light C17H17N5O6S2 MW: 451
Source:
Synthetic
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