CF dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. CF dyes are Biotiums line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red/near-IR fluorescent CF680 dye has excitation/emission at 681/698 nm.
Source:
Synthetic
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