Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 94.7 kDa. The protein migrates as 110-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human EGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human EGF, Tag Free at 10 ug/ml (100 uL/well) can bind Human EGF R, Fc Tag with a linear range of 0.078-2.5 ug/ml.
Immobilized Human EGF R, Fc Tag at 1 ug/ml (100 uL/well) can bind Cetuximab with a linear range of 0.1-2 ng/mL.
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