Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 30-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Cynomolgus/Rhesus macaque CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) at 5 ug/ml (100 uL/well) can bind Cynomolgus/Rhesus macaque CD47, His Tag with a linear range of 4-63 ng/mL.
Immobilized Anti-CD47, Human IgG4 at 2 ug/ml (100 uL/well) can bind Cynomolgus/Rhesus macaque CD47, His Tag with a linear range of 0.2-5 ng/mL.
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