Rabbit JDP2 Recombinant Monoclonal Antibody, Clone: [BLR246L], Unconjugated

Catalog Number: BYT-ORB1733245
Article Name: Rabbit JDP2 Recombinant Monoclonal Antibody, Clone: [BLR246L], Unconjugated
Biozol Catalog Number: BYT-ORB1733245
Supplier Catalog Number: orb1733245
Alternative Catalog Number: BYT-ORB1733245-10,BYT-ORB1733245-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, ICC, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Between 50 and 100
Conjugation: Unconjugated
Alternative Names: jun dimerization protein 2, JUNDM2, progesterone receptor co-activator
JDP2 Antibody
Clonality: Recombinant
Concentration: 400 µg/ml
Clone Designation: [BLR246L]
NCBI: 569736
UniProt: Q8WYK2
Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Form: Liquid
Target: JDP2
Application Dilute: Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10 6 cells. , Application : ICC , ApplicationDilutionRange : 1:40 to 1:100. Epitope retrieval with Tris-EDTA pH 9.0 is recommend
Application Notes: Application Notes: Format: Whole IgG
Detection of mouse JDP2 (shaded) in CTLL-2 cells by flow cytometry. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human JDP2 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human JDP2 by immunocytochemistry. Sample: FFPE section of human FaDu cells. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human JDP2 by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 by immunohistochemistry. Sample: FFPE section of mouse RenCa tumor. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human JDP2 by immunohistochemistry. Sample: FFPE section of human spleen. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and RenCa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human JDP2 by western blot. Samples: Whole cell lysate (25 µg) from MCF-7, HeLa, HaCaT, and DU145 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.