Phospho-B-Raf (Ser602) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB4717
- Images (8)
| Article Name: | Phospho-B-Raf (Ser602) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB4717 |
| Supplier Catalog Number: | orb4717 |
| Alternative Catalog Number: | BYT-ORB4717-50,BYT-ORB4717-100,BYT-ORB4717-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P |
| Species Reactivity: | Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human B-Raf around the phosphorylation site of Ser602 VK(p-S)RW |
| Conjugation: | Unconjugated |
| Alternative Names: | BRaf (p-S602), p-BRaf, phospho-BRaf, B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1, 9930012E13Rik, Braf-2, Braf2, C230098H17, D6Ertd631e, BRAF_HUMAN, BRAF, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, 2.7.11.1, BRAF_MOUSE, |
| Phospho-B-Raf (Ser602) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 94 kDa |
| UniProt: | P15056 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | BRAF |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Application Notes: | Modification: Phosphorylated |
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IHC-P of rat brain tissue using B-Raf (phospho-Ser602) antibody. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Ser602)) Polyclonal Antibody, Unconjugated (orb4717) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Ser602)) Polyclonal Antibody, Unconjugated (orb4717) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Ser602)) Polyclonal Antibody, Unconjugated (orb4717) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Ser602)) Polyclonal Antibody, Unconjugated (orb4717) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Ser602)) Polyclonal Antibody, Unconjugated (orb4717) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Ser602)) Polyclonal Antibody, Unconjugated (orb4717) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-B-Raf (Ser602) Polyclonal Antibody, Unconjugated (orb4717) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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