Gremlin Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB500806
- Images (8)
| Article Name: | Gremlin Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB500806 |
| Supplier Catalog Number: | orb500806 |
| Alternative Catalog Number: | BYT-ORB500806-50,BYT-ORB500806-100,BYT-ORB500806-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human gremlin (101-184/184aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | C15DUPq, CKTSF1B1, CRAC1, CRCS4, DAND2, DRM, DUP15q, GREMLIN, HMPS, HMPS1, IHG-2, MPSH, PIG2, Grem, ld, GREM1_HUMAN, GREM1, Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, BMP antagonist 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, Increased in high glucose protein 2 (IHG-2), GREM1_MOUSE, GREM1_RAT, |
| Gremlin Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 21 kDa |
| UniProt: | O60565 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | GREM1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Gremlin) Polyclonal Antibody, Unconjugated (orb500806) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat rectum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Gremlin) Polyclonal Antibody, Unconjugated (orb500806) at 1:200 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Gremlin) Polyclonal Antibody, Unconjugated (orb500806) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: intestine (Mouse) Lysate at 40 ug, skin (Mouse) Lysate at 40 ug, Primary: Anti-Gremlin at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 24 kD. |
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Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-Gremlin (orb500806) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD. |
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Tissue/Cell: Mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Gremlin Polyclonal Antibody, Unconjugated (orb500806) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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IHC-P analysis of Mouse embryo tissue using Anti-Gremlin Polyclonal Antibody at 1:200 dilution. |
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IHC-P analysis of rat rectum using Gremlin Polyclonal Antibody at 1:200 dilution. |
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