Phospho-AKT (Ser473) Mouse Monoclonal Antibody, Clone: [9H11], Unconjugated
Catalog Number:
BYT-ORB506119
- Images (8)
| Article Name: | Phospho-AKT (Ser473) Mouse Monoclonal Antibody, Clone: [9H11], Unconjugated |
| Biozol Catalog Number: | BYT-ORB506119 |
| Supplier Catalog Number: | orb506119 |
| Alternative Catalog Number: | BYT-ORB506119-50,BYT-ORB506119-100,BYT-ORB506119-200 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human AKT around the phosphorylation site of Ser473 QF(p-S)YS |
| Conjugation: | Unconjugated |
| Alternative Names: | AKT1 (p-S473), p-AKT1, phospho-AKT1, AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, LTR-akt, PKB/Akt, PKBalpha, O57513_CHICK, akt1, RAC-PK-alpha, 2.7.11.1, AKT1_HUMAN, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, AKT1_MOUSE, AKT1 kinase, Thymoma viral proto-oncogene, AKT1_RAT, |
| Phospho-AKT (Ser473) Mouse Monoclonal Antibody |
| Clonality: | Monoclonal |
| Concentration: | 1mg/ml |
| Clone Designation: | [9H11] |
| Molecular Weight: | 56 kDa |
| UniProt: | P31749 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | AKT1 |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:500-1000, IF=1:500-1000 |
| Application Notes: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (ascites of orb506119 9H11) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (orb506119) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human skin carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (ascites of orb506119 9H11) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (ascites of orb506119 9H11) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (orb506119) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (orb506119) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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IHC-P analysis of mouse brain using phospho-AKT Ser473 Monoclonal Antibody at 1:200 dilution. |
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IHC-P analysis of rat brain using phospho-AKT Ser473 Monoclonal Antibody at 1:200 dilution. |
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