HSD3B1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB5478
Article Name: HSD3B1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB5478
Supplier Catalog Number: orb5478
Alternative Catalog Number: BYT-ORB5478-50,BYT-ORB5478-100,BYT-ORB5478-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human HSD3B1 (301-373/373aa)
Conjugation: Unconjugated
Alternative Names: 3BETAHSD, HSD3B, HSDB3, HSDB3A, SDR11E1, 3-beta-HSD I, D3Ertd383e, 3BHS1_HUMAN, HSD3B1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I (3-beta-HSD I), 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxysteroid 3-dehydrogenase, Delta-5-3-ketosteroid isomerase, Dihydrotestosterone oxidoreductase, Steroid Delta-isomerase, Trophoblast antigen FDO161G, 3BH, 3BHS2_MOUSE, Hsd3b2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II (3-beta-HSD II), 3BHS1_RAT,
HSD3B1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 41 kDa
Pubmed: 31175881
UniProt: P14060
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: HSD3B1
Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (mouse ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
IHC-P analysis of Rat liver using HSD3B1 Polyclonal Antibody at 1:400 dilution.
IHC-P analysis of mouse placenta using HSD3B1 Polyclonal Antibody at 1:200 dilution.