Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody, Clone: [2H5], Unconjugated
Catalog Number:
BYT-ORB559074
- Images (8)
| Article Name: | Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody, Clone: [2H5], Unconjugated |
| Biozol Catalog Number: | BYT-ORB559074 |
| Supplier Catalog Number: | orb559074 |
| Alternative Catalog Number: | BYT-ORB559074-50,BYT-ORB559074-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human Cytokeratin 5 (550-590aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CK5, DDD, DDD1, EBS1, EBS2, EBS2A, EBS2B, EBS2C, EBS2D, EBS2E, EBS2F, K5, KRT5A, 3300001P10Rik, Krt2-5, Tfip8, CK-5, Sak57, K2C5_HUMAN, KRT5, 58 kDa cytokeratin, Cytokeratin-5 (CK-5), Keratin-5 (K5), Type-II keratin Kb5, K2C5_MOUSE, K2C5_RAT, |
| Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [2H5] |
| Molecular Weight: | 64 kDa |
| UniProt: | P13647 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | KRT5 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:200-2000, IHC-F=1:200-2000, IF=1:200-2000 |
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Paraformaldehyde-fixed, paraffin embedded (Human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 5) Monoclonal Antibody, Unconjugated (orb559074) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse trachea), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: human A431 cell lysates, Primary: Anti-Cytokeratin 5 (orb559074) at 1/500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1/5000 dilution, Predicted band size: 64 kD, Observed band size: 62 kD. |
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IHC-P analysis of Human skin cancer using Cytokeratin 5 Polyclonal Antibody at 1:200 dilution. |
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IHC-P analysis of mouse trachea using Cytokeratin 5 Polyclonal Antibody at 1:200 dilution. |
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