Immunofluorescence staining of U251 cells with CSB-PA001268EA01HU at 1:160,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA001268EA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA001268EA01HU at dilution of 1:100
Western blot All lanes: ADA antibody at 2µg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa
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