ADA Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA001268EA01HU
Article Name: ADA Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA001268EA01HU
Supplier Catalog Number: CSB-PA001268EA01HU
Alternative Catalog Number: CSB-PA001268EA01HU-100UG, CSB-PA001268EA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: ada antibody, ADA_HUMAN antibody, ADA1 antibody, Adenosine aminohydrolase antibody, Adenosine deaminase antibody
Clonality: Polyclonal
UniProt: P00813
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: ADA
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunofluorescence staining of U251 cells with CSB-PA001268EA01HU at 1:160,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA001268EA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA001268EA01HU at dilution of 1:100
Western blot
All lanes: ADA antibody at 2µg/ml + Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa