COX5A Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA00414A0RB
Article Name: COX5A Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA00414A0RB
Supplier Catalog Number: CSB-PA00414A0Rb
Alternative Catalog Number: CSB-PA00414A0RB-100UG, CSB-PA00414A0RB-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: COX antibody, COX VA antibody, COX5A antibody, COX5A_HUMAN antibody, Cytochrome c oxidase polypeptide Va antibody, Cytochrome c oxidase polypeptide, mitochondrial antibody, Cytochrome c oxidase subunit 5A antibody, Cytochrome c oxidase subunit 5A, mitochondrial antibody, Cytochrome c oxidase subunit Va antibody, mitochondrial antibody, Mitochondrial cytochrome c oxidase subunit Va antibody, VA antibody
Clonality: Polyclonal
UniProt: P20674
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: COX5A
Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunofluorescence staining of Hela cells with CSB-PA00414A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA00414A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human glioma using CSB-PA00414A0Rb at dilution of 1:100
Western blot
All lanes: COX5A antibody at 2µg/ml + 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa