YARS Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA00465A0RB
Article Name: YARS Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA00465A0RB
Supplier Catalog Number: CSB-PA00465A0Rb
Alternative Catalog Number: CSB-PA00465A0RB-100UG, CSB-PA00465A0RB-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: CMTDIC antibody, SYYC_HUMAN antibody, Tyrosine tRNA ligase, cytoplasmic antibody, Tyrosine tRNA ligase 1, cytoplasmic antibody, Tyrosyl tRNA synthetase antibody, Tyrosyl--tRNA ligase antibody, Tyrosyl-tRNA synthetase, cytoplasmic antibody, TyrRS antibody, yars antibody, YRS antibody, YTS antibody
Clonality: Polyclonal
UniProt: P54577
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: YARS
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00465A0Rb at dilution of 1:100
Western blot
All lanes: YARS antibody at 2µg/ml + 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Immunofluorescence staining of HepG2 cells with CSB-PA00465A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of SH-SY5Y cells with CSB-PA00465A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA00465A0Rb at dilution of 1:100