GPR75 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA009829LA01HU
Article Name: GPR75 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA009829LA01HU
Supplier Catalog Number: CSB-PA009829LA01HU
Alternative Catalog Number: CSB-PA009829LA01HU-100UG, CSB-PA009829LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: G protein coupled receptor 75 antibody, GPR chr2 antibody, Gpr75 antibody, GPR75_HUMAN antibody, GPRchr2 antibody, OTTHUMP00000159608 antibody, Probable G protein coupled receptor 75 antibody, Probable G-protein coupled receptor 75 antibody, WI 31133 antibody, WI31133 antibody
Clonality: Polyclonal
UniProt: O95800
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: GPR75
Application Dilute: Recommended dilution: WB:1:500-1:2000, IF:1:20-1:100
Immunohistochemistry of paraffin-e
Immunofluorescence staining of A549 cell with CSB-PA009829LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg), SY5Y whole cell lysate(30µg)
All lanes: GPR75 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 120s