Acetyl-H2AFZ (K7) Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA010100OA07ACHU
- Images (5)
| Article Name: | Acetyl-H2AFZ (K7) Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA010100OA07ACHU |
| Supplier Catalog Number: | CSB-PA010100OA07acHU |
| Alternative Catalog Number: | CSB-PA010100OA07ACHU-100UL, CSB-PA010100OA07ACHU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, ELISA, ICC, IF, IP, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | H2A histone family member Z antibody, H2A.z antibody, H2A/z antibody, H2afz antibody, H2AZ antibody, H2AZ_HUMAN antibody, Histone H2A.Z antibody, MGC117173 antibody |
| Clonality: | Polyclonal |
| UniProt: | P0C0S5 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | H2AFZ |
| Application Dilute: | Recommended dilution: WB:1:200-1:2000, ICC:1:20-1:200, IF:1:20-1:200, IP:1:200-1:2000 |
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Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFZ (CSB-PA010100OA07acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter. |
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Immunofluorescence staining of Hela cells with CSB-PA010100OA07acHU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate (All treated by 30mM sodium butyrate for 4h) All lanes: H2AFZ antibody at 0.48µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa |
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Immunocytochemistry analysis of CSB-PA010100OA07acHU diluted at 1:50 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating H2AFZ in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) Lane 1: Rabbit control IgG instead of CSB-PA010100OA07acHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA010100OA07acHU (3µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg) Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg) |
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