Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity:
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form:
liquid
Target:
AKT1
Application Dilute:
WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt(Phospho-Thr308) Antibody.
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt (Phospho-Thr308) Antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody.
Western blot analysis of extracts from Hela cells untreated or treated with EGF, serum or both using Akt(Phospho-Thr308) Antibody.
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