The antiserum was produced against synthesized peptide derived from human Annexin A6. AA range:1-50
Alternative Names:
ANXA6, ANX6, Annexin A6, 67 kDa calelectrin, Annexin VI, Annexin-6, Calphobindin-II, CPB-II, Chromobindin-20, Lipocortin VI, Protein III, p68, p70
Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010],
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Immunofluorescence analysis of Hela cell. 1,Annexin VI Polyclonal Antibody(green) was diluted at 1:200(4 overnight). (red) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:RS3608 was diluted at 1:1000(room temperature, 50min).
Western Blot analysis of various cells using Annexin VI Polyclonal Antibody diluted at 1:2000
Western Blot analysis of 293 cells using Annexin VI Polyclonal Antibody diluted at 1:2000
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