Phospho-AKT1 (S124) (4J11) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE05846
Product Overview
Article Name:
Phospho-AKT1 (S124) (4J11) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE05846
Supplier Catalog Number:
AMRe05846
Alternative Catalog Number:
EKL-AMRE05846-50UL, EKL-AMRE05846-100UL, EKL-AMRE05846-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
IP, WB
Species Reactivity:
Human, Mouse, Rat
Conjugation:
Unconjugated
Alternative Names:
AKT 1, PKB, PKB-ALPHA, PRKBA, Protein Kinase B Alpha, Protein kinase B, Proto-oncogene c-Akt, RAC Alpha,
Product Description
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis (PubMed:15526160, PubMed: 11882383, PubMed: 21620960, PubMed: 21432781). This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates (PubMed: 15526160, PubMed: 11882383, PubMed: 21620960, PubMed: 21432781). Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported (PubMed: 15526160, PubMed: 11882383, PubMed: 21620960, PubMed: 21432781). AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface (By similarity). Phosphorylation of PTPN1 at Ser-50 negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling (By similarity). Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport (PubMed: 11994271). AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at Ser-21 and GSK3B at Ser-9, resulting in inhibition of its kinase activity (By similarity). Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven (By similarity). AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase) (PubMed: 11154276). Phosphorylation of Ser-83 decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis (PubMed: 11154276). AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at Ser-939 and Thr-1462, thereby activating mTORC1 signaling and leading to both phosphorylation of 4E- BP1 and in activation of RPS6KB1 (PubMed: 12150915). AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization (PubMed: 10358075). In particular, FOXO1 is phosphorylated at Thr-24, Ser-256 and Ser-319 (PubMed: 10358075). FOXO3 and
Product Properties
Clonality:
Monoclonal
Molecular Weight:
56kDa
NCBI:
207
UniProt:
P31749
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
AKT1
Application Notes
Application Dilute:
WB 1:500-1:2000,IP 1:50-1:200
