Phospho-KAP1 (S824) (13F9) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE05933
Product Overview
Article Name:
Phospho-KAP1 (S824) (13F9) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE05933
Supplier Catalog Number:
AMRe05933
Alternative Catalog Number:
EKL-AMRE05933-50UL, EKL-AMRE05933-100UL, EKL-AMRE05933-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
IP, WB
Species Reactivity:
Human, Mouse
Conjugation:
Unconjugated
Alternative Names:
KAP 1, KRAB associated protein 1, KRIP1, RNF96, TF1B, TIF1 beta, TIF1B, Trim28, Tripartite motif containing 28,
Product Description
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at Lys-9 (H3K9me)) to the promoter regions of KRAB target genes. Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at Lys-9 (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 Lys-9 and Lys-14 acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation, the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed: 24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed: 24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed: 24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3-exons of zinc- finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed: 27029610). Acts as a corepressor for ZFP568 (By similarity).
Product Properties
Clonality:
Monoclonal
Molecular Weight:
89kDa
NCBI:
10155
UniProt:
Q13263
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
TRIM28
Application Notes
Application Dilute:
WB 1:500-1:2000,IP 1:10-1:100
