Phospho-PKR (T446) (4N9) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE05976
Product Overview
Article Name:
Phospho-PKR (T446) (4N9) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE05976
Supplier Catalog Number:
AMRe05976
Alternative Catalog Number:
EKL-AMRE05976-50UL, EKL-AMRE05976-100UL, EKL-AMRE05976-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
IP, WB
Species Reactivity:
Human
Conjugation:
Unconjugated
Alternative Names:
E2AK2,E2AK2, EIF2AK1, EIF2AK2, MGC126524, PKR p68 kinase, PKR, PRKR,
Product Description
PKR a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. IFN-induced dsRNA-dependent serine/threonine-protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) and plays a key role in the innate immune response to viral infection (PubMed:18835251, PubMed: 19507191, PubMed: 19189853, PubMed: 21123651, PubMed: 21072047, PubMed: 22948139, PubMed: 23229543, PubMed: 22381929). Inhibits viral replication via the integrated stress response (ISR): EIF2S1/eIF-2- alpha phosphorylation in response to viral infection converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, resulting to a shutdown of cellular and viral protein synthesis, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4 (PubMed: 19189853, PubMed: 21123651, PubMed: 22948139, PubMed: 23229543). Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1) (PubMed: 11836380, PubMed: 19189853, PubMed: 20171114, PubMed: 19840259, PubMed: 21710204, PubMed: 23115276, PubMed: 23399035). Also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation: phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11 (PubMed: 11836380, PubMed: 22214662, PubMed: 19229320). In addition to serine/threonine- protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at Tyr-4 upon DNA damage, facilitating its ubiquitination and proteosomal degradation (PubMed: 20395957). Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs (PubMed: 22948139, PubMed:
Product Properties
Clonality:
Monoclonal
Molecular Weight:
62kDa
NCBI:
5610
UniProt:
P19525
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
EIF2AK2
Application Notes
Application Dilute:
WB 1:500-1:2000,IP 1:10-1:20
