Brd4 (2D15) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE07650
Product Overview
Article Name:
Brd4 (2D15) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE07650
Supplier Catalog Number:
AMRe07650
Alternative Catalog Number:
EKL-AMRE07650-50UL, EKL-AMRE07650-100UL, EKL-AMRE07650-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
FC, ICC, IHC, IP, WB
Species Reactivity:
Human, Mouse, Rat
Conjugation:
Unconjugated
Alternative Names:
Brd4, CAP, HUNK1, MCAP, Bromodomain containing 4, chromosome associated protein,
Product Description
Bromodomain-containing protein 4 (BRD4) is a member of the bromodomains and extra terminal (BET) family of proteins, which also includes BRD2, BRD3, and BRDT. BET family proteins contain two tandem bromodomains and an extra terminal (ET) domain, and bind acetyl lysine residues. BRD4 is a chromatin-binding protein with a preference for Lys14 on histone H3 as well as Lys5 and Lys12 on histone H4. BRD4 chromatin binding occurs throughout the cell cycle, including condensed mitotic chromosomes, when the majority of genes are silenced. Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure (PubMed:23589332, PubMed: 23317504, PubMed: 22334664). During interphase, plays a key role in regulating the transcription of signal- inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6. BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II (PubMed: 23589332, PubMed: 19596240, PubMed: 16109377, PubMed: 16109376, PubMed: 24360279). Promotes phosphorylation of Ser-2 of the C-terminal domain (CTD) of RNA polymerase II (PubMed: 23086925). According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of Ser-2 of the C-terminal domain (CTD) of RNA polymerase II, these data however need additional evidences in vivo (PubMed: 22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B (PubMed: 19103749). Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters (PubMed: 23317504).
Product Properties
Clonality:
Monoclonal
Molecular Weight:
152kDa
NCBI:
23476
UniProt:
O60885
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
BRD4
Application Notes
Application Dilute:
WB 1:500-1:2000,IHC 1:50-1:200,ICC/IF 1:100-1:200,FC 1:20-1:50,IP 1:20-1:50
