Caspase-8 (3Q3) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE07982
Product Overview
Article Name:
Caspase-8 (3Q3) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE07982
Supplier Catalog Number:
AMRe07982
Alternative Catalog Number:
EKL-AMRE07982-50UL, EKL-AMRE07982-100UL, EKL-AMRE07982-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
IF, IHC, WB
Species Reactivity:
Human, Mouse, Rat
Conjugation:
Unconjugated
Alternative Names:
Caspase 8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, FADD-homologous ICE/ced-3-like protease, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACH, Caspase-8 subunit p18, CAP4,
Product Description
Caspases are a family of cytosolic aspartate specific cysteine proteases. Involved in the activation cascade of caspases responsible for apoptosis execution. Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (By similarity). Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death (PubMed:23516580, PubMed: 8681376, PubMed: 8681377, PubMed: 9006941, PubMed: 9184224, PubMed: 8962078). Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (PubMed: 8962078, PubMed: 9006941). Binding to the adapter molecule FADD recruits it to either receptor TNFRSF6/FAS mediated or TNFRSF1A (PubMed: 8681376, PubMed: 8681377). The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation (PubMed: 9184224). The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (PubMed: 9184224). Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (PubMed: 9184224). In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at Asp-324, which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (PubMed: 31827280, PubMed: 31827281). Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-D (GSDMD): GSDMD cleavage promoting release of the N-terminal moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (By similarity). Initiates pyroptosis following inactivation of MAP3K7/TAK1 (By similarity). Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (By similarity). May participate in the Granzyme B (GZMB) cell death pathways (PubMed: 8755496). Cleaves PARP1 (PubMed: 8681376).
Product Properties
Clonality:
Monoclonal
Molecular Weight:
55kDa
NCBI:
841
UniProt:
Q14790
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
CASP8
Application Notes
Application Dilute:
WB 1:1000-1:5000,IHC 1:100-1:200,IF-P 1:100-1:200
