Human POLd (Polymerase DNA Directed Delta 1) ELISA Kit
Biozol Catalog Number:
EKL-EH26882
Supplier Catalog Number:
EH26882
Alternative Catalog Number:
EKL-EH26882-96T
Manufacturer:
EnkiLife
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
CDC2, POLD1, DNA polymerase subunit delta p125
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human POLd. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human POLd and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human POLd, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human POLd. You can calculate the concentration of Human POLd in the samples by comparing the OD of the samples to the standard curve.
Range:
0.15-10ng/mL
Sensitivity:
0.054ng/mL
Samples:
Serum,plasma and other biological fluids
Target:
POLd
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