CD256, TALL2, TALL-2, TNFSF13, APRIL, APRILFLJ57090, TRDL1, TRDL-1, ZTNF2, 2310026N09Rik
Recombinant Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.It contains Lys112-Leu250 trimer design.
Molecular Weight:
The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Lys112-Leu250
Target:
APRIL/TNFSF13
Immobilized Cynomolgus APRIL (Trimer), His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA (QC Test).
Cynomolgus APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus APRIL Trimer is greater than 95% as determined by SEC-HPLC.
Loaded Cynomolgus APRIL (Trimer), His Tag on Anti-His-Biosensor can bind Human TACI, hFc Tag with an affinity constant of 4.06 nM as determined in BLI assay (Gator Prime).
Loaded Cynomolgus APRIL (Trimer), His-Avi Tag on Anti-His-Biosensor can bind Cynomolgus BCMA, hFc Tag with an affinity constant of 1.59 nM as determined in BLI assay (Gator Prime).
* VAT and and shipping costs not included. Errors and price changes excepted
