CD256, TALL2, TALL-2, TNFSF13, APRIL, APRILFLJ57090, TRDL1, TRDL-1, ZTNF2, 2310026N09Rik
Recombinant Human APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Lys112-Leu250 trimer design.
Molecular Weight:
The protein has a predicted MW of 49.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Lys112-Leu250
Target:
APRIL/TNFSF13
Human APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human APRIL Trimer is greater than 95% as determined by SEC-HPLC.
Immobilized Human APRIL Trimer, His Tag at 0.1 ug/ml (100 ul/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 0.9 ng/ml determined by ELISA (QC Test).
Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Human APRIL (Trimer), His-Flag Tag with an affinity constant of 0.76nM as determined in BLI assay (Gator Prime).
Loaded Human BCMA, hFc Tag on ProA-Biosensor can bind Human APRIL (Trimer), His Tag with an affinity constant of 0.24 nM as determined in BLI assay (Gator Prime).
Immobilized Human APRIL Trimer, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APRIL Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
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