Recombinant Human/Cynomolgus Activin RIIB/ACVR2B Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus).
Molecular Weight:
The protein has a predicted MW of 14.75 kDa. Due to glycosylation, the protein migrates to 25-45 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus)
Target:
Activin RIIB/ACVR2B
Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC.
Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A-with an affinity constant of 27.92 pM as determined in SPR assay (Biacore T200).
Immobilized Human Activin A, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3 ng/ml determined by ELISA (QC Test).
Immobilized Human/Cynomolgus Activin RIIB, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Bimagrumab with the EC50 of 4.3 ng/ml determined by ELISA.
Serial dilutions of Bimagrumab were added into Human/Cynomolgus Activin RIIB, His Tag : Biotinylated Human Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml.
Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GDF-8, Avi Tag with the EC50 of 2.7ng/ml determined by ELISA.
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