Recombinant Human/Cynomolgus Activin RIIB/ACVR2B Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus).
Molecular Weight:
The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus)
Target:
Activin RIIB/ACVR2B
The purity of Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC.
Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 0.5ng/ml determined by ELISA.
Immobilized Human/Cynomolgus Activin RIIB, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Activin RIIB Antibody, hFc Tag with the EC50 of 24.7ng/ml determined by ELISA.
Immobilized Human Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
Human/Cynomolgus Activin RIIB, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF-2, No Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
Immobilized Human Latent GDF-11, His Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.
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